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. 2015 May 5;7(5):957–968. doi: 10.1080/19420862.2015.1045168

Figure 5.

Figure 5.

TTAC-0001 inhibits angiogenesis in vitro. (a) TTAC-0001 inhibits VEGF-mediated proliferation of HUVEC. HUVECs were incubated with TTAC-0001 (1, 15 and 20 µg/ml) in the media containing VEGF (10 ng/ml). ** p-value < 0.01 versus VEGF alone treated cells. (b) TTAC-0001 inhibits VEGF-mediated migration of HUVEC. HUVECs were incubated with TTAC-0001 (20 µg/ml) for 0.5 hour and plated onto modified Boyden Chambers, and allowed to migrate in response to 10 ng/ml of VEGF for 3.5 hours at 37 °C. cells were stained with crystal violet. (c) TTAC-0001 inhibits in vitro tube formation. HUVECs were seeded onto matrigels and cultured in the VEGF containing media without TTAC-0001 or with 5, 10 and 20 µg of TTAC-0001. 20 µg of 6C1 was also treated as a negative control.