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Activity of intracellular antibody LL3 in the HEK293 bioassay. HEK293 luciferase bioassay cells expressing VHH intracellular antibody were stimulated with LiCl2 (A) or Wnt3a-conditioned media (B). Firefly luciferase activity was measured and plotted as fold stimulation over unactivated cells, 5 replicates were performed for each condition. Results are representative of 2 independent experiments. **** denotes p ≤ 0.0001 for a 2 tailed unpaired Student's t-test. Data shows mean and SEM.
