Table 2.

Produced β-catenin fragments

Fragment description	Amino acids	Primer sequences used in construction	MW (kDa)
F1. N-terminus and first α helix	1–163	GTCACAGGATCCATGGCTACTCAAGCTGATTTGATGG CAGGTTGTCGACTCATTACTCGTCATTTAGCAGTTTTGTCAGTTCAGG	22.0
F2. N-terminus and first 6 armadillo repeats	1–390	GTCACAGGATCCATGGCTACTCAAGCTGATTTGATGG CAGGTTGTCGACTCATTATGCAGCATCTGAAAGATTCCTG	46.7
F3. C-terminus and last 6 armadillo repeats	391–781	GGTAAGCAGGATCCGCTGCAACTAAACAGGAAGG CAGGTTGTCGACTCATTACAGGTCAGTATCAAACCAGGCC	46.8
F4. All 12 armadillo repeats and C-terminus	138–781	CTGGTAAGCAGGATCCAACTTGATTAACTATCAAGATGATGCAGAACTTGCC CAGGTTGTCGACTCATTACAGGTCAGTATCAAACCAGGCC	74
F5. Full length	1–781	GTCACAGGATCCATGGCTACTCAAGCTGATTTGATGG CAGGTTGTCGACTCATTACAGGTCAGTATCAAACCAGGCC	89.5