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. 2015 Mar 11;7(2):390–402. doi: 10.1080/19420862.2015.1007818

Figure 7.

Figure 7.

Analysis of apoptosis induced by the EMI405(scFv)-ETA' immunotoxin fusion protein. (A) Flow cytometry was used to detect apoptotic cells by annexin A5-FITC/PI double-staining. Kasumi‑1 cells were treated with 1 μg EMI405(scFv)-ETA', non-binding 425(scFv)-ETA' or PBS and analyzed after incubating 4 24 h. Viable cells are shown in the lower left quadrant, early apoptotic cells in the lower right quadrant and late apoptotic/necrotic cells in the upper right quadrant. PtdIns: Propidium Iodide. (B) Histogram shows percent apoptosis based on activation of caspases-3/7 in Kasumi‑1 cells (gray bars) and KG‑1 control cells (white bars) after subtracting background (incubation with PBS). The cells were treated with 1 μg EMI405(scFv)-ETA' for up to 96 h. *** highly significant.