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. 2015 Jan 27;10(1):6–18. doi: 10.4161/15592294.2014.988035

Figure 2.

Figure 2.

MS275 regulates both expression and localization of ARHGEF3 in leukemia. (A) Analysis of ARHGEF3 expression levels in U937 cells upon MS275 treatment (5 μM) at the indicated times by RT-PCR. The standard deviation was calculated from experiments in biological triplicate. (B) Expression levels of ARHGEF3 in U937 cells after MS275 treatment (5 μM) at the indicated times by Western blot analysis. ERK was used as loading control. (C) ChIP assays were performed in U937 cells treated with MS275 at the indicated times using H3K9,14 ac antibody. The analysis shows the recovery (% IP/Input) of H3K9,14 ac on ARHGEF3 promoter region. (D) IF analysis of ARHGEF3 showing its localization in U937 cells treated with MS275 for the indicated times. Cells were fixed and immune-stained with anti-ARHGEF 3 (red). Nuclear DNA was stained with Hoechst (blue). Scale bar 10 μm.