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. 2015 Jan 27;10(1):6–18. doi: 10.4161/15592294.2014.988035

Figure 5.

Figure 5.

MS275 affects Elk phosphorylation by RhoA pathway activation. (A) Western blot analysis of expression levels of p-JNK, p-p38 and p-Elk1 in U937 cells after MS275 treatment (5 μM) at the indicated times with or without exoenzyme C3 transferase (1 μg/mL). ERK was used as loading control. (B) Protein expression levels of p-Elk1 and p-ERK in U937 cells after MS275 treatment (5 μM) with or without the MEK inhibitor U0126 (10 μM) for the indicated times. ERK was used as loading control. (C) Protein expression levels of p-Elk1 in U937 cells after MS275 treatment (5 μM) with or without the ROCK inhibitor Y27632 (10 μM) at the indicated times. ERK was used as loading control. (D) Schematic model showing MS275-induced ARHGEF3 involvement in activation of RhoA pathway by phosphorylation of JNK and its target Elk1. (E) Western blot analysis of expression levels of ARHGEF3 in U937 cells after MS275 treatment (5 μM) at the indicated times with or without CEP1347 (0.5 μM). β-actin was used as loading control.