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. 2015 Apr 18;16(5):770–779. doi: 10.1080/15384047.2015.1026477

Figure 2.

Figure 2.

p8 upregulation decreases the sensitivity of tumor cells to dihydroartemisinin. After transfection with pCCL− p8 plasmid or specifically targeted p8 siRNA for 24 h, (A) HeLa and HCT116 cells were treated with serial concentrations of DHA for 48 h, and then cell survival was detected by SRB assay. (B) HeLa and HCT116 were treated with 20 μM DHA for 24 h, after which PARP, caspase-3 and cleaved-caspase-3 protein levels were measured by western blot analysis. (C-F) siRNA or plasmid-transfected HeLa cells treated with 20 μM DHA for 24 h, and quantification of apoptosis was performed using Annexin V/PI or Annexin V-PE/7-AAD followed by FACS analysis. Three independent experiments were performed and the values were expressed as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.