Figure 4.

Transgenic expression of CitMTSE1 in antisense orientation in orange fruits confers different levels of resistance against fungal and bacterial specialized pathogens. (A, B) Progress of the disease caused by the fungus Penicillium digitatum in mature orange fruits inoculated with 1×10⁴ spores mL⁻¹. (A) Percentage of infected wounds in orange fruits from AS6, AS7 and EV lines. The results are the average ± SEM (n ≥ 20). dpi, days postinoculation. (B) AS and EV fruits at 6 dpi. (C, D) Progress of the disease caused by the bacterium Xanthomonas citri subsp citri in green orange fruits inoculated with 10⁶ CFU mL⁻¹. (C) Percentage of infected wounds in orange fruits from AS6, AS7 and EV lines at 4 weeks postinoculation (wpi). The results are the average ± SEM (n ≥ 10). (D) AS6 and EV fruits at 4 wpi. We repeated all experiments several times during 2 consecutive seasons and obtained similar results. For each time point, means with different letter are significantly different according to Fisher's Protected LSD test (P ≤ 0.05) applied after an ANOVA to arcsine-transformed data.