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. 2015 May 21;7(4):732–742. doi: 10.1080/19420862.2015.1045173

Table 2.

Quantitative evaluation of method performance. Each analytical method was applied in 2 separate series 6 replicates per batch. Relative abundance of the various glycan species are given in percent, with standard deviations in parentheses. Key: H, hexose; N, N-acetylhexosamine; F, deoxyhexose; S, N-acetylneuraminic acid (sialic acid); G0F-N, agalacosylated, core-fucosylated, monoantennary species, etc.; n.d.: not detected; n.a.: not applicable. n,q.: not quantifiable

Glycan species Structural scheme HILIC(2-AB)Reference Method ESI-MS Heavy Chain ESI-MS after IdeS ESI-MS Glycopeptides LCMS with Orbitrap LCMS with Q-TOF nano-LCMS with Q-TOF PGC-MS Positive MALDI_MS Glycopeptides Negative MALDI-MS Glycopeptides MALDI-MS Glycans MALDI-MS Stabilized Glycans
G0F[H3N4F1] graphic file with name kmab-07-04-1045173-i001.gif 35.5 (0.1)35.3 (0.1) 34.9 (0.2)34.7 (0.2) 31.3 (0.5)32.0 (0.3) 29.3 (0.2)30.6 (0.7) 29.8 (0.2)32.4 (0.3) 29.7 (0.4)29.9 (0.3) 32.4 (0.5)33.6 (0.9) 36.5 (0.4)36.2 (0.2) 37.8 (0.9)38.2 (0.3) 38.0 (0.9)38.4 (0.1) 37.3 (0.2)37.6 (0.5) 30.6 (0.7)31.2 (0.2)
G1F[H4N4F1] graphic file with name kmab-07-04-1045173-i002.gif 43.4 (0.1)43.3 (0.1) 45.5 (0.2)45.6 (0.2) 39.5 (0.3)39.6 (0.3) 40.6 (1.0)38.6 (0.4) 38.4 (0.1)39.2 (0.3) 40.1 (0.4)40.1 (0.3) 42.4 (0.3)40.5 (1.8) 42.7 (0.4)42.6 (0.2) 46.6 (0.5)46.5 (0.1) 47.0 (0.5)46.9 (0.2) 44.4 (0.6)44.4 (0.6) 46.1 (0.7)47.9 (0.3)
G2F[H5N4F1] graphic file with name kmab-07-04-1045173-i003.gif 9.5 (<0.1)9.6 (0.1) 10.7 (0.1)11.1 (0.1) 11.9 (0.5)11.1 (0.3) 9.8 (0.2)8.6 (0.4) 10.1 (0.1)9.3 (0.1) 9.0 (0.1)9.0 (0.1) 9.7 (0.3)9.0 (0.3) 9.2 (0.1)9.2 (0.1) 7.3 (0.5)7.3 (0.2) 7.0 (0.4)7.0 (0.1) 7.0 (0.1)6.6 (0.3) 9.9 (0.5)9.0 (0.3)
G0[H3N4] graphic file with name kmab-07-04-1045173-i004.gif 4.6 (0.1)4.7 (0.1) 9.0 (0.3)8.5 (0.2) 6.0 (0.1)6.0 (0.1) 5.1 (0.3)5.9 (0.3) 6.1 (<0.1)6.6 (<0 .1) 4.4 (0.1)4.6 (0.1) 4.9 (0.3)5.8 (0.5) 3.6 (0.1)3.8 (0.1) 3.6 (0.2)3.8 (<0.1) 3.4 (0.1)3.7 (0.1) 5.0 (0.1)4.8 (0.2) 4.3 (0.2)4.0 (0.2)
G1[H4N4] graphic file with name kmab-07-04-1045173-i005.gif 3.3 (<0.1)3.4 (<0 .1) n.d.n.d. n.q.n.q. 3.7 (0.2)3.8 (0.1) 4.7 (0.1)4.8 (<0.1) 3.0 (0.1)3.0 (0.2) 3.3 (0.2)3.6 (0.2) 2.5 (<0.1)2.6 (<0.1) 2.1 (0.1)2.0 (0.1) 2.0 (0.1)1.9 (<0.1) 3.0 (0.1)3.0 (0.1) 4.1 (0.1)3.8 (0.1)
G2[H5N4] graphic file with name kmab-07-04-1045173-i006.gif 0.3 (<0 .1)0.4 (<0.1) n.d.n.d. n.d.n.d. 1.1 (0.1)1.0 (0.1) 0.7 (<0 .1)0.6 (0.1) 0.4 (<0 .1)0.4 (<0.1) 0.9 (0.1)0.9 (0.1) 0.5 (<0 .1)0.4 (<0.1) 0.3 (<0 .1)0.2 (<0.1) 0.3 (<0 .1)0.2 (<0.1) 0.5 (0.1)0.5 (0.1) 1.5 (0.1)1.3 (0.2)
G0F-N[H3N3F1] graphic file with name kmab-07-04-1045173-i007.gif 0.5 (<0.1)0.5 (<0.1) n.d.n.d. 1.1 (0.1)1.2 (0.1) 2.7 (0.2)3.7 (0.2) 2.5 (0.1)1.2 (<0.1) 5.6 (0.1)5.6 (0.1) 1.0 (<0.1)0.9 (<0.1) 0.5 (<0.1)0.5 (<0.1) 0.4 (<0.1)0.3 (<0.1) 0.4 (<0.1)0.3 (<0.1) 0.6 (0.1)0.8 (0.1) <0.1 (<0.1)<0.1 (<0.1)
G1F-N[H4N3F1] graphic file with name kmab-07-04-1045173-i008.gif n.d.(n.d.) n.d.n.d. 2.4 (0.1)2.6 (0.1) 2.7 (0.2)2.4 (0.1) 1.3 (<0.1)0.9 (<0.1) 2.7 (0.1)2.6 (0.1) 0.7 (<0.1)0.6 (<0.1) 0.6 (<0.1)0.6 (<0.1) 0.3 (<0.1)0.2 (<0.1) 0.3 (<0.1)0.2 (<0.1) 0.5 (<0.1)0.4 (<0.1) 0.5 (<0.1)0.4 (0.1)
G0-N[H3N3] graphic file with name kmab-07-04-1045173-i009.gif 0.4(<0.1)0.4(<0.1) n.d.n.d. 1.1 (0.1)1.1 (0.1) 1.1 (0.1)1.3 (0.1) 1.2 (<0.1)0.9 (<0.1) 1.3 (0.1)0.9 (0.1) 0.7 (<0 .1)0.7 (0.1) 0.2 (<0.1)0.2 (<0.1) 0.2 (<0.1)0.2 (<0.1) 0.2 (<0.1)0.2 (<0.1) 0.8 (0.1)1.0 (<0.1) 0.2 (0.1)0.1 (<0.1)
M5[H5N2] graphic file with name kmab-07-04-1045173-i010.gif 1.5 (<0.1)1.6 (<0.1) n.d.n.d. 2.4 (0.1)2.3 (0.1) 1.9 (0.2)1.9 (<0.1) 2.9 (0.1)3.1 (0.1) 1.2 (0.1)1.3 (0.1) 1.9 (<0.1)1.9 (0.1) 0.5 (<0.1)0.5 (<0.1) 1.1 (0.1)1.0 (<0.1) 1.1 (0.1)1.0 (<0.1) 0.9 (<0.1)0.9 (0.1) 1.0 (0.1)0.9 (0.2)
M6[H6N2] graphic file with name kmab-07-04-1045173-i011.gif 0.1 (<0.1)0.1 (<0.1) n.d.n.d. n.d.n.d. 0.4 (0.1)0.3 (0.1) 0.2 (<0.1)0.2 (<0.1) 0.1 (<0.1)0.1 (<0.1) n.d.n.d. n.d.n.d. n.q.n.q. n.q.n.q. n.d.n.d. n.d.n.d.
G1FS[H4N4FS1] graphic file with name kmab-07-04-1045173-i012.gif 0.2 (<0.1)0.2 (<0.1) n.d.n.d. 1.3 (0.1)1.4 (0.1) 0.4 (0.1)0.4 (<0 .1) 0.4 (<0.1)0.1 (<0.1) 0.4 (<0.1)0.3 (<0.1) n.d.n.d. 0.9 (<0.1)0.9 (<0.1) n.q.n.q. n.q.n.q. n.d.n.d. 0.3 (0.1)0.2 (<0.1)
G2S1F[H5N4FS1] graphic file with name kmab-07-04-1045173-i013.gif 0.7 (<0.1)0.7 (0.1) n.d.n.d. 1.9 (0.2)1.8 (0.1) 0.6 (0.1)0.6 (0.1) 1.1 (<0 .1)0.4 (<0.1) 0.9 (0.1)0.6 (0.1) 1.4 (0.1)1.5 (0.1) 2.1 (0.1)2.2 (0.1) n.q.n.q. n.q.n.q. n.d.n.d. 0.7 (0.1)0.5 (<0.1)
G2S2F[H5N4FS2] graphic file with name kmab-07-04-1045173-i014.gif 0.1 (<0.1)0.1(<0.1) n.d.n.d. 1.0 (0.1)1.0 (0.1) 0.2 (0.1)0.2 (<0.1) 0.2(<0.1)<0.1 (<0.1) 0.3 (<0.1)0.3 (<0.1) 0.4 (0.1)0.5 (0.1) n.d.n.d. n.d.n.d. n.d.n.d. n.d.n.d. 0.3 (0.1)0.1 (0.1)