Figure 1.

Amino acid sequences of adalimumab variable heavy (VH) and light chain (VL) regions and the design of the combinatorial histidine substitution libraries. Amino acid sequence alignments show the VH (A) and VL (B) regions of adalimumab and variants with pH-dependent antigen binding (PSV#1, PSV#2 and PSV#3). CDRs are indicated in black boxes. Distribution of histidines substitutions within CDRs of the VH (C) and VL (D) libraries were calculated from 89 and 80 analyzed sequences, respectively. Genes used for sequencing were also used for the generation of yeast surface display libraries. Mutations with respect to the parental adalimumab sequences are shown with dots indicating identical residues.