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. 2015 Jan 14;10(2):167–177. doi: 10.1080/15592294.2014.998536

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© 2015 The Author(s). Published with license by Taylor & Francis Group, LLC

© Clara Serra-Juhé, Ivon Cuscó, Aïda Homs, Raquel Flores, Núria Torán, and Luis A Pérez-Jurado

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License http://creativecommons.org/licenses/by-nc/3.0/, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 3. — Hypermethylation at one CpG located in the TRR of MTHFS in case iCHD_4. A: Relative MTHFS mRNA in heart tissue of the case and controls by qRT-PCR. Data were normalized such that the level of another control was 1.0 represented by the gray line. The results represent the mean +/− SD of 2 independent experiments conducted in triplicate. B: Relative MTHFS mRNA in heart tissue of the case and one control by semiquantitative analysis using agarose gel. Two transcripts of MTHFS were quantified using RIMBP2 as a control gene. The results represent the mean +/− SD of 3 independent experiments.