Figure 3.

Consecutive binding of IL-1α and IL-1β to ABT-981. To generate this representative sensogram, ABT-981 was immobilized on a Biacore sensor chip via goat anti-human IgG and 500 nM human IL-1β was injected followed by injection of 500 nM human IL-1α. No dissociation time of the IL-1β was allowed prior to the injection of the IL-1α. The sensogram indicates that human IL-1β binds to ABT-981, and IL-1α variable domains are still accessible for binding to IL-1α. The reciprocal of this study (binding of IL-1α followed by IL-1β) showed identical results (data not shown). The Y-axis of the plot is response units and the X-axis is time in seconds.