Table 3.

PCR and pyrosequencing primers

NR3C1 1F Assay 2 – CpG sites 5–1 - chr5:143,404,013–143,404,147*
PCR primer forward- Biotinylated	/5Biosg/ GTTGTTATTAGTAGGGGTATTGG
PCR primer reverse	AACCACCCAATTTCTCCAATTTCTTTTC
Pyrosequencing primer (reverse)	CAACTCCCCCACTCCAAACCC
NR3C1 1F Assay 1 – CpG sites 6–10 - chr5:143,404,011–143,404,097*
PCR primer forward	AGTTTTAGAGTGGGTTTGGAG
PCR primer reverse-Biotinylated	/5Biosg/ AAAACCACCCAATTTCTCCAATTTCTT
Pyrosequencing primer (forward)	GAGTGGGTTTGGAGT
BDNF IV – CpG sites 1–5 - chr11:27,701,519–27,701,826*
PCR primer forward	GGGTTGGAAGTGAAAATATTTGTAAA
PCR primer reverse-Biotinylated	/5Biosg/CCCCATCAACCAAAAACTCCATTTAATCTC
Pyrosequencing primer (forward)	GGTAGAGGAGGTATTATATGATAG

^*Genomic coordinates show genomic regions amplified by PCR and are based on the UCSC Genome Browser Human Dec. 2013 (GRCh38/hg38) Assembly.