Protodermal areas of seedlings treated with NOA (A–C), NAA + TIBA (D and E) and NAA + NOA (F). The asterisks mark advanced GMCs and the arrowheads in (B) and (E) point to SMC nucleus. (A–C) Optical sections of stomatal rows after aniline blue staining (A and C) and under DIC optics (B). Note the absence of subsidiary cells adjacent to advanced GMCs (A, B) and to young guard cells (C). (D and E) Stomatal row of NAA + TIBA-treated leaves containing advanced GMCs (D) and young stomata (E). Note the absence of subsidiary cells. (F) Protodermal region of seedlings incubated with NAA + NOA lacking subsidiary cells. Treatments: (A–C): NOA 50 μM, 72 h; (D and E): NAA 100 μM + TIBA 300 μM, 72 h; (F): NAA 100 μM + NOA 50 μM, 72 h. Scale Bars: 10 μm.