Figure 4.

FGFR4-silencing inhibited the migration of CNE2 cells. A: Silenced and control cells were grown until confluence and their migratory capabilities were analyzed by wound-healing assay. Representative images of the wound-healing assay are shown at 0 and 48 h with a microscope (× 200 magnifications). Migration speed of the cells was calculated by the wound width/the distance measured at 0 h. B: The histogram showed the relative migration distance of cells. C: Transwell assay was used to show the penetration of FGFR4-silencing cells through the membrane compared with the control. D: Absolute number of cells that migrated through the member. The data were means± SEM. * P <0.05.