Figure 5.

FGFR4 knockdown inhibited the inducement of EMT in CNE2 cells. A: FGFR4-silencing and the control cells were lysed and subjected to Western blot analysis using specific antibodies against the EMT markers. B: The bar demonstrated the expression ratio of the target protein to β-actin by densitometry. The data shown were representative of at least three independent experiments. Data were analyzed by Student's t-test.*P < 0.05. C: Immunofluorescence analysis of EMT markers in CNE2 cells transfected with either FGFR4-siRNA or control siRNA. DAPI was used for counterstaining the nucleus in blue. E-Cadherin, N-cadherin and Vimentin staining was in red.