Figure 5.

Plant 2G12 SIgA retains antigen binding and is capable of aggregating lab-adapted strains of HIV. (A) Functional ELISA showing binding of 2G12 antibodies expressed in plants and CHO cells to baculovirus-expressed HIV gp120 (clade IIIB) (B) Calculated particle size and relative volume concentration for BaL virions (gray line) in the presence of specific and non-specific control IgGs; CHO 2G12 IgG (black dots and dashes), Nt-derived 2G12 IgG (black line) and polyclonal IgG from human sera (gray dashes). Volume concentration is given by (# particles × 10⁶) / ml (C) As (B), comparing aggregation in the presence of P2G12 SIgA (black line) and non-specific SIgA from human colostrum (black dots and dashes).