Binding activity of AIA22 to different antigens. All the antigens were coated at 5 μg/ml. The optical density (OD) at 492 nm reduced in a dose-dependent manner with decreasing amounts of AIA22 when binding to IFN-α1b and IFN-α2b. No cross-reactivity was observed when AIA22 was incubated with other type I IFN (IFN-β, IFN-ω), type II IFN (IFN-γ), cytokines (VEGF, TNF), and human serum albumin (HSA).