Figure 4.

TUNEL and caspase-3 immunohistochemistry in MCF-7 cells transfected with UGT2B4, 2B7, and 2B15. Cells were transfected with the indicated UGT2B expression plasmid or Lipofectamine 2000 alone as a sham control. Forty-eight hours after transfection, TUNEL and activated caspase-3 assays were performed as described in Methods and visualized by fluorescence microscopy using 200 x magnification. Nuclei were visualized by DAPI stain. For quantification, 10 independent fields were analyzed. Bar graphs depict the average quantification of imaged fluorescence signals from TUNEL and caspase-3-positive cells and vertical lines indicate SEM, n=4. Transfection were compared against the sham control for statistical significance; *= p < 0.05; **=p < 0.01.