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. 2014 Oct 30;6(6):1621–1630. doi: 10.4161/19420862.2014.975098

Figure 1.

Figure 1.

Potency of Hu 15C1 at blocking TLR4 is influenced by Fc-FcγR cis interaction (A) Dose response inhibition of LPS-induced TLR4 activation in THP1-Xblue cells. The potency of Hu 15C1 alone (black circles), after adding IVIg (gray squares), after blocking FcγRII (black triangles), after adding IVIg and blocking FcγRII (black crosses), after adding IVIg and blocking FcγRIIB (black diamonds), were compared to the potency of the F(ab)’2 Hu 15C1 (open circles). Results are expressed as mean ± SD of duplicates. An F test was used to compare the fitted curves of different groups. The p values are presented in the supplementary Table S1. (B) The IC50 of F(ab)’2 Hu 15C1 (black bars) and Hu 15C1 (white bars) at blocking LPS-induced TLR4 activation in U937 cells plated at high (left panel; U937 high) or low confluence (right panel; U937 low). Results are expressed as mean ± SD of 2 independent experiments. An F test was used to compare the different groups. ***P < 0.001, ns: not significant. Bar on photomicrograph represents 20 microns.