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. 2014 Oct 30;6(6):1621–1630. doi: 10.4161/19420862.2014.975098

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© 2014 The Author(s). Published with license by Taylor & Francis Group, LLC

© Jérémy Loyau, Pauline Malinge, Bruno Daubeuf, Limin Shang, Greg Elson, Marie Kosco-Vilbois, Nicolas Fischer, and François Rousseau

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 4. — Levels of TLR4 and FcγRs expressed at the cell surface of THP1 cells (A) The expression levels of TLR4, FcγRI, and FcγRII at the surface of THP1-Xblue cells as measured by flow cytometry; isotype control (grey); for TLR4, FcγRI and FcγRII; Hu 15C1, 10.1 and IV.3 antibodies were used, respectively (black). (B) The expression level of FcγRIIb at the surface of THP1-Xblue cells as measured by flow cytometry; isotype control (grey); Hu 15C1 (black). (C) Receptor number on THP1-Xblue cells as determined using calibration beads and flow cytometry. The receptors densities were measured in duplicate with cells at passage 5 and 15. The variations between measurements are indicated by the error bars.