Figure 9.

TET1 oxidates 5mC preferably at highly methylated promoters. The promoters showing the highest de novo or fold change increase of 5hmC (A) and those promoters characterized by a complete loss or lowest fold change of 5hmC upon TET1 overexpression (B) are grouped according to their basal, endogenous methylation levels as determined by the HumanMethylation450 BeadChip experiments. Comparisons between 5hmC and HumanMethylation450 data were only performed for genes with identical gene symbol annotations in both data sets (top de novo promoters: n = 470, top fold change promoters: n = 477, complete loss promoters: n = 237, lowest fold change promoters: n = 424).