Figure 3.

Consistent associations between RFS and the MELK genes are observed across the training data set and validating data set. Kaplan–Meier survival plot for RFS for MELK indicates increased expression (red) of this gene indicates poor prognosis in the training dataset (A) and validating data set (B). Breast cancer patients grouped by the median of gene expression level, significances were assessed by logrank test. (D) Log2 transformed mRNA expression values of MELK in MCF-7 tumor xenografts treated with either tamoxifen or control. P values were calculated by independent 2-tailed t test. Error bars represent mean ± SD. (E) mRNA expression values of MELK in tamoxifen resistant/sensitive MCF-7 subclones treated with tamoxifen or control. P values were calculated by unpaired 2-tailed t test. Error bars represent mean ± SD.