Figure 3.

Cell-Type-Specific Sensorimotor Processing during the Detection Task

(A) A D1-Cre mouse was crossed with a LSL-tdTomato mouse, driving expression of tdTomato in dSPNs (upper left). The recorded neuron was filled with biocytin and stained with Alexa-647 (upper middle). The labeled neuron was considered as a dSPNs because the Alexa-647 signal co-localized with tdTomato (upper right). Average hit and miss traces from the example positively identified dSPN (below).

(B) iSPNs express tdTomato in an A2A-Cre × LSL-tdTomato mouse (upper left). The recorded neuron was filled with biocytin and stained with Alexa-647 (upper middle). The biocytin stain co-localized with tdTomato defining this neuron as an iSPN (upper right). Average hit and miss traces (below) from the example positively identified iSPN.

(C) Grand average V_m of hit trials across all positively identified dSPNs (black, n = 5 cells) and iSPNs (blue, n = 5 cells), showing an early sensory response specifically in dSPNs. Lighter shaded lines represent SEM.

(D) The slope of the early response was significantly larger in dSPNs versus iSPNs (n = 5 of each cell type, Wilcoxon Mann-Whitney two-sample rank test, p = 0.008).

(E) The amplitude of the early response (Early ΔV_m), was significantly larger for dSPNs versus iSPNs (n = 5 of each cell type, Wilcoxon Mann-Whitney two-sample rank test, p = 0.03).

(F) The late response (Late ΔV_m) was not significantly different in dSPNs versus iSPNs (n = 5 of each cell type, Wilcoxon Mann-Whitney two-sample rank test, p = 1).

Values are mean ± SEM. ^∗p < 0.05; ^∗∗p < 0.01; ns, non-significant. See also Figure S3.