Figure 1.

B Cell-Specific WIP Deficiency Compromises Humoral Immune Responses

JHT-WT or JHT-Wipf1^−/− mixed BM chimeric mice were infected intra footpad with Vaccinia virus (A) or immunized intra-peritoneally with NP-KLH in alum (B–D).

(A) Analysis of immune responses in popliteal lymph nodes at day 8 post-infection by flow cytometry. GC cells (B220⁺CD95⁺GL7⁺) or Tfh cells (CD4⁺CD44⁺CD62L⁻PD-1⁺CXCR5⁺) are shown. Quantifications (right column) indicate the percentage of GC B cells or Tfh cells per lymph node analyzed (mean ± SEM).

(B and C) Analysis of splenic NP-specific GC cells (NP⁺B220⁺CD95⁺GL7⁺) at day 13 post-immunization by flow cytometry. Immunohistochemistry showing IgD⁺ B cell follicles, Bcl-6 expressing GC cells and TCR-β⁺ T cell areas in frozen spleen sections. Graph indicates quantifications of GC cells in spleen sections (mean ± SEM). Scale bar, 150 μm.

(D) ELISA of NP-specific IgM, IgG, IgG1, and IgG3 antibodies in the sera of immunized chimeras at indicated time points (mean ± SEM).

Data are representative of two independent experiments (eight mice each).

See also Figure S1.