Figure 4.

WIP Regulates PI3K Signaling and Mediates Class-Switching and Plasma Cell Formation In Vitro

(A and B) Immunoblot of splenic WT or Wipf1^−/− B cells stimulated with CD40L (A) or LPS (B), probed with antibodies as indicated. Quantifications of intensity of proteins normalized by densitometry to Erk or tubulin, and to the signal in unstimulated WT cells at t = 0. This experiment and experiment in Figure S4D were performed simultaneously. The loading control measurements were part of both experiments.

(C and D) CTV labeled splenic WT and Wipf1^−/− B cells were stimulated for 4 days with CD40L (C) or LPS (D) and IL-4 and IL-5. Flow cytometry measurements of proliferation (CTV dilution, upper row), class-switch recombination (IgG1⁺ cells, middle row) and plasma cell differentiation (CD138^hi cells, lower row). Graphs on the right of (C) and (D) indicate the percentages of class-switched and PCs normalized to total live cells. Data are pooled from at least three experiments (mean ± SEM).

See also Figure S4.