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. 2015 Mar 4;10(3):e992707. doi: 10.4161/15592324.2014.992707

Figure 2.

Figure 2.

Elevated pre-dawn expression of GA biosynthetic genes in elf3 mutants requires PIF4 and PIF5. Transcript abundance for (A) GA20ox1, (B) GA20ox2, (C) GA20ox3, (D) GA20ox4, (E) GA20ox5 and (F) GA3ox1 in the indicated genotypes at 15 minutes before ZT0 (lights-on) under SD conditions. Transcript levels were measured by qPCR analysis. Genotypes are depicted on x-axis and y-axis displays relative quantitation (RQ) of transcript normalized to PDF2 according to Materials and Methods. Shown is the mean (±SEM) of 3 independent experimental replicates.