Figure 1.

Metformin selectively induces apoptosis in 3 human gastric cancer cell lines, but not the noncancerous one. (A): 48 h after addition of 5 mM metformin, caspase 3/7 activities were measured with Caspase-Glo assay as described in Materials and methods. Cells without metformin treatment were served as the control. Data are shown as mean (Rectangular box) ± SD (Error bar) and represent 3 independent experiments. (B): 48 h after 5 mM metformin treatment, cleavages of PARP and caspase 3 were examined by protein gel blotting, β-actin was probed as the loading control. Panel B is the representative image of 3 independent experiments.