Table 1.
Similarity of camelid IGV genes to human IGV counterparts
| Canonical Fold |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| A | Camelid IGHV | # seq | Source | Human IGHV | % Identity (FRs) | Human | Camelid | ||
| Lama pacos (Alpaca) | V1 | IGHV1-1*01 to IGHV1-3 | F | 5 | WGS Ref | IGHV1-2*02 | 81–92 | 1–2 | 1–2 |
| IGHV1-1*02; IGHV1-4 | P | 2 | WGS Ref | IGHV1-2*02 | ND | 1–2 | ND | ||
| V3 | IGHV3 | F | 45 | WGS Ref | IGHV3-23*04 | 86–94 | 1–3 | 1–3 | |
| IGHV3-7; IGHV3-8; IGHV3-10 | P | 3 | WGS Ref | IGHV3-23*04 | ND | 1–3 | ND | ||
| IGHV3-17 | F | 2 | WGS Ref | IGHV3-66*02 | 88–92 | 1–1 | 1–1 | ||
| IGHHV3-1; IGHHV3-s14 | F | 4 | WGS Ref | IGHV3-30*04 | 82–84 | 1–3 | 1–3 | ||
| IGHHV3-2 to IGHHV3-4; s2-7 | F | 6 | WGS Ref | IGHV3-11 | 82–88 | 1–3 | 1–3 | ||
| IGHHV3-5 to IGHHV3-7; s15 | F | 5 | WGS Ref | IGHV3-66*02 | 80–84 | 1–1 | 1–1 | ||
| V4 | IGHV4-1 to IGHV4-11 | F | 8 | WGS Ref | IGHV4-30-4*01 | 80–89 | 3–1 | 3–1 | |
| IGHV4-12 to IGHV4-15 | F | 5 | WGS Ref | IGHV4-30-4*01 | 77–82 | 3–1 | 3-x | ||
| V5 | IGHV5-1 | P | 1 | WGS | IGHV5-51 | ND | 1–2 | ND | |
| V7 | IGHV7-1 | P | 1 | WGS | IGHV7-4 | ND | 1–2 | ND | |
| Camelus ferus | V1 | IGHV1-1 to IGHV1-4 | P | 4 | WGS | IGHV1-2*02 | ND | 1–2 | ND |
| V3 | IGHV3-1 to IGHV3-3 | F | 3 | WGS | IGHV3-23*04 | 86–93 | 1–3 | 1–3 | |
| IGHV3-4 to IGHV3-5 | P | 2 | WGS | IGHV3-23*04 | ND | 1–3 | ND | ||
| IGHV3-6 | F | 1 | WGS | IGHV3-66*02 | 95 | 1–1 | 1–1 | ||
| IGHHV3-1 to IGHHV1-3 | F | 3 | WGS | IGHV3-66*02 | 67–74 | 1–1 | 1–1 | ||
| IGHHV3-4 to IGHHV1-5 | P | 2 | WGS | IGHV3-66*02 | ND | 1–3 | ND | ||
| V4 | IGHV4-1 | F | 1 | WGS | IGHV4-30-4*01 | 86 | 3–1 | 3–1 | |
| IGHV4-1 to IGHV3 | P | 2 | WGS | IGHV4-30-4*01 | ND | 3–1 | ND | ||
| V5 | IGHV5-1 | F | 1 | WGS | IGHV5-51 | 78 | 1–2 | 1–2 | |
| V7 | IGHV7-1 | P | 1 | WGS | IGHV7-4 | ND | 1–2 | ND | |
|
Canonical Fold |
|||||||||
| B | Camelid IGLV | #seq | Source | Human IGLV | % Identity (FRs) | Human | Camelid | ||
| Lama pacos (Alpaca) | V1 | IGLV1-1*01-*02 | F | 2 | WGS HTG | IGLV1-47*02 | 85.50 | 13–7 | 13–7 |
| IGLV1-2 to 1-6 | F | 8 | WGS HTG | IGLV1-40*01 | 86–90 | 14–7 | 14–7 | ||
| IGLV1-7 to 1-11 | P | 5 | WGS | IGLV1-40*01 | ND | 14–7 | ND | ||
| V2 | IGLV2-1 to 2-5 | F | 6 | WGS | IGLV2-18*01 | 83–88 | 14–7 | 14–7 | |
| IGLV2-6 to 2-11 | P | 6 | WGS | IGLV2-18*01 | ND | 14–7 | ND | ||
| V3 | IGLV3-1 to 3-3 | F | 3 | WGS | IGLV3-9*01 | 87–91 | 11–7 | 11–7 | |
| IGLV3-4 to 3-15 | F | 12 | WGS | IGLV3-25*02/ *03 | 81–87 | 11–7 | 11–7 | ||
| IGLV3-16 to 3-25 | P | 10 | WGS | ND | ND | 11–7 | ND | ||
| V4 | IGLV4-1 | F | 1 | WGS | IGLV4-60*03 | 68 | 12–11 | 12–11 | |
| IGLV4-2 to 4-7 | P | 6 | WGS | IGLV4-60*03 | ND | 12–11 | ND | ||
| V5 | IGLV5-1 to 5-9 | F | 9 | WGS HTG | IGLV5-39*01; 45*01, 37*01, 52*01 | 69–91 | 14–11 | 14–11 | |
| IGLV5-10 | F | 1 | HTG | IGLV5-37*01 | 84 | 14–11 | 13–11 | ||
| IGLV5-11 to 5-17 | P | 8 | WGS HTG | IGLV5-37*01 | ND | 14–11 | ND | ||
| V6 | IGLV6-1 | P | 2 | WGS HTG | IGLV6-57*01 | ND | 13–7 | ND | |
| V7 | IGLV7-1 | NA | NA | NA | NA | NA | |||
| V8 | IGLV8-1 to 8-10 | F | 10 | WGS HTG | IGLV8-61*01 | 81–91 | 14–7 | 14–7 | |
| IGLV8-11 to 8-14 | P | 7 | WGS HTG | IGLV8-61*01 | ND | 14–7 | ND | ||
| V9 | IGLV9-1*01-*02 | F | 2 | WGS HTG | IGLV9-49*01 | 65 | 12–12 | 12–12 | |
| IGLV9-2 to 5 | P | 4 | WGS | IGLV9-49*01 | ND | 12–12 | ND | ||
| V10 | IGLV10-1 | F | 1 | WGS HTG | IGLV10-54*02 | 71 | 13–7 | 13–7 | |
| Camelus ferus | V1 | IGLV1-1 | F | 1 | WGS | IGLV1-47*02 | 81 | 13–7 | 13–7 |
| IGLV1-2 to 1-7 | F | 6 | WGS | IGLV1-40*01 | 66–90 | 14–7 | 14–7 | ||
| IGLV1-8 to 1-15 | P | 8 | WGS | IGLV1-40*01 | ND | 14–7 | ND | ||
| V2 | IGLV2-1 to 2-5 | F | 7 | WGS | IGLV2-18*01 | 81–88 | 14–7 | 14–7 | |
| IGLV2-6 to 2-9 | P | 4 | WGS | IGLV2-18*01 | ND | 14–7 | ND | ||
| V3 | IGLV3-1 | F | 1 | WGS | IGLV3-9*01 | 91 | 11–7 | 11–7 | |
| IGLV3-2 to 3-6 | F | 5 | WGS | IGLV3-25*02/*03 | 81–88 | 11–7 | 11–7 | ||
| IGLV3-7 to 3-9 | P | 3 | WGS | ND | ND | 11–7 | ND | ||
| V4 | IGLV4-1 to 4-2 | F | 2 | WGS | IGLV4-60*03 | 70–74 | 12–11 | 12–11 | |
| IGLV4-3 to 4-5 | P | 3 | WGS | IGLV4-60*03 | ND | 12–11 | ND | ||
| V5 | IGLV5-1 to 5-10 | F | 9 | WGS | IGLV5-39*01; 45*01, 37*01, 52*01 | 79–86 | 14–11 | 14–11 | |
| IGLV5-11 to 5-19 | P | 9 | WGS | IGLV5-37*01 | ND | 14–11 | ND | ||
| V6 | IGLV6-1 | P | 1 | WGS | IGLV6-57*01 | ND | 13–7 | ND | |
| V7 | IGLV7-1 | F | 1 | WGS | IGLV7-46*01 | 80 | 14–7 | 14–7 | |
|
Table 1. Similarity of camelid IGV genes to human IGV counterparts | |||||||||
|
Canonical Fold |
|||||||||
| B | Camelid IGLV | #seq | Source | Human IGLV | % Identity (FRs) | Human | Camelid | ||
| V8 | IGLV8-1 to 8-7 | F | 7 | WGS | IGLV8-61*01 | 81–87 | 14–7 | 14–7 | |
| IGLV8-8 to 8-11 | P | 4 | WGS | IGLV8-61*01 | ND | 14–7 | ND | ||
| V9 | IGLV9-1 to 9-3 | P | 3 | WGS | IGLV9-49*01 | ND | 12–12 | ND | |
| V10 | IGLV10-1 to 10-2 | F | 2 | WGS | IGLV10-54*02 | 69–71 | 13–7 | 13–7 | |
| Lama glama (llama) | V1 | IGLV1-1 | F | 1 | PCR | IGLV1-47*02 | 84 | 13–7 | 13–7 |
| IGLV1-2 to 1-6 | F | 5 | PCR | IGLV1-40*01 | 84–91 | 14–7 | 14–7 | ||
| IGLV1-7 to 1-12 | P | 6 | PCR | IGLV1-40*01 | ND | ND | ND | ||
| V2 | IGLV2-1 to 2-5 | F | 7 | PCR | IGLV2-18*01 | 84–88 | 14–7 | 14–7 | |
| IGLV2-6 to 2-7 | P | 2 | PCR | IGLV2-18*01 | ND | 14–7 | ND | ||
| V3 | IGLV3-1 to 3-3 | F | 4 | PCR | IGLV3-9*01 | 87–88 | 11–7 | 11–7 | |
| IGLV3-4 to 3-11 | F | 8 | PCR | IGLV3-25*02/*03 | 81–87 | 11–7 | 11–7 | ||
| V4 | IGLV4-1 to 4-2 | F | 2 | PCR | IGLV4-60*03 | 70–75 | 12–11 | 12–11 | |
| IGLV4-3 to 4-7 | P | 5 | PCR | IGLV4-60*03 | ND | 12–11 | ND | ||
| V5 | IGLV5-1 to 5-10 | F | 10 | PCR | IGLV5-39*01; 45*01, 37*01, 52*01 | 75–90 | 14–11 | 14–11 | |
| IGLV5-11 | F | 1 | PCR | IGLV5-37*01 | 84 | 14–11 | 13–11 | ||
| IGLV5-12 to 5-20 | P | 8 | PCR | ND | ND | 14–11 | ND | ||
| V6 | IGLV6-1 | P | 2 | PCR | IGLV6-57*01 | ND | 13–7 | ND | |
| V7 | IGLV7-1 | F | 1 | PCR | IGLV7-43*01 | 84 | 14–7 | 14–7 | |
| V8 | IGLV8-1 to 8-4 | F | 4 | PCR | IGLV8-61*01 | 87–88 | 14–7 | 14–7 | |
| IGLV8-5 to 8-8 | P | 4 | PCR | IGLV8-61*01 | ND | 14–7 | ND | ||
| V9 | IGLV9-1 to 9-3 | F | 3 | PCR | IGLV9-49*01 | 65–68 | 12–12 | 12–12 | |
| V10 | IGLV10-1*01-*02 | F | 2 | WGS | IGLV10-54*02 | 69–71 | 13–7 | 13–7 | |
|
Canonical Fold |
|||||||||
| C | Camelid IGKV | #seq | Source | Human IGKV | % Identity (FRs) | Human | Camelid | ||
| Lama pacos | V1 | IGKV1-1 to 1-2 | F | 2 | HTG ; WGS | IGKV1-39*01 | 84–86 | 2–1 | 2–1 |
| IGKV1-3 to 1-4 | F | 3 | HTG ; WGS | IGKV1-27*01 | 80–86 | 2–1 | 2–1 | ||
| IGKV1-5*01-*02 | P | 2 | HTG ; WGS | IGKV1-27*01 | ND | 2–1 | ND | ||
| V2 | IGKV2-1 to 2-5 | F | 5 | HTG ; WGS | IGKV2D-29*02 | 83 | 4–1 | 4–1 | |
| IGKV2-6*01-*02 | F | 2 | HTG ; WGS | IGKV2-40*01 | 60–61 | 3–1 | 3–1 | ||
| V3 | IGKV3-1 to 3-2 | F | 3 | HTG ; WGS | IGKV3-11*01 | 60–61 | 2–1 | 2–1 | |
| IGKV3-3 to 3-4 | P | 2 | WGS | IGKV3-11*01 | ND | 2–1 | ND | ||
| V4 | IGKV4-1 to 4-2 | F | 3 | HTG ; WGS | IGKV4-1*01 | 78–80 | 3–1 | 3–1 | |
| V5 | IGKV5-1 | F | 1 | HTG ; WGS | IGKV5-2*01 | 76 | 2–1 | 2–1 | |
| V6 | IGKV6-1 | F | 1 | WGS | IGKV6D-41*01 | 77 | 2–1 | 2–1 | |
| Camelus ferus | V1 | IGKV1-1 to 1-2 | F | 2 | WGS | IGKV1-39*01 | 80 | 2–1 | 2–1 |
| IGKV1-3 | F | 1 | WGS | IGKV1-27*01 | 80 | 2–1 | 2–1 | ||
| IGKV1-3 to 1-6 | P | 3 | WGS | IGKV1-27*01 | ND | 2–1 | ND | ||
| V2 | IGKV2-1 | F | 1 | WGS | IGKV2D-29*01 | 83 | 4–1 | 4–1 | |
| IGKV2-2 to 2-4 | P | 3 | WGS | IGKV2D-29*01 | ND | 4–1 | ND | ||
| V3 | IGKV3-1 to 3-2 | F | 1 | WGS | IGKV3-11*01 | 60–62 | 2–1 | 2–1 | |
| V4 | IGKV4-1 to 4-2 | F | 2 | WGS | IGKV4-1*01 | 78–80 | 3–1 | 3–1 | |
| V5 | IGKV5-1 | F | 1 | WGS | IGKV5-2*01 | 77 | 2–1 | 2–1 | |
| V6 | IGKV6-1 | F | 1 | WGS | IGKV6D-41*01 | 77 | 2–1 | 2–1 | |
| Lama glama | V1 | IGKV1-1 | F | 1 | PCR | IGKV1-27*01 | 81 | 2–1 | 2–1 |
| V2 | IGKV2-1 to 2-4 | F | 10 | PCR | IGKV2D-29*01 | 82 | 4–1 | 4–1 | |
| IGKV2-5 | F | 1 | PCR | IGKV2-40*01 | 60 | 3–1 | 3–1 | ||
| V4 | IGKV4-1 | F | 1 | PCR | IGKV4-1*01 | 81 | 3–1 | 3–1 | |
| V5 | IGKV5-1 | F | 1 | PCR | IGKV5-2*01 | 73 | 2–1 | 2–1 | |
| V6 | IGKV6-1*01-02 | F | 2 | PCR | IGKV6D-41*01 | 77 | 2–1 | 2–1 | |
Camelid IGV germline families were compared to their human counterparts based on the canonical folds of CDR1-2 and the identity of their corresponding frameworks (FRs). The first 5 columns (camelid IGV, #seq and Source) indicate Lama pacos and Camelus ferus IGHV (A), IGLV (B) and IGKV (C) families along with their corresponding members. The numbers of sequences (#seq) are based on all sequences including allelic versions (*) of the families found similar to the human IGV gene counterparts (sixth column). The last 3 columns summarize the range of identity of the camelid frameworks and canonical folds of CDR1 and CDR2 compared to human counterparts. ND indicates genes from which the % identity and the canonical folds could not to be determined. The letters P and F indicate camelid pseudo genes and functional IGV family members, respectively. One-to-one IGHV, IGLV and IGKV comparison, including sequence ID and Genebank accession numbers, is illustrated in Table S1.