Table 2.
X-ray crystallography, data collection and refinement statistics
| Data collection | Anti-CD70, 27B3 | Anti-MET, 48A2 |
|---|---|---|
| Beamline | Proxima 1 | Proxima 1 |
| Space group | P212121 | P212121 |
| Cell dimensions (Å/°) | 63.6, 66.9, 125.4 | 107.0, 121.5, 185.9 |
| Resolution limitsa (Å) | 65.0–1.75 (1.85–1.75) | 45.0–3.3(3.4–3.3) |
| Rmeasa (%) | 7.4 (60) | 12.0 (58.6) |
| No of observationsa | 344853 (56009) | 120361 (8817) |
| No unique reflectionsa | 55037 (8761) | 35909 (2593) |
| Mean((I)/sd(I))a | 16.4 (3.0) | 7.0 (2.4) |
| Completenessa (%) | 99.9 (99.5) | 97.5 (97.1) |
| Multiplicitya | 6.1 (6.3) | 3.3 (3.4) |
| Refinement | ||
| No of Fabs molecules | 1 | 4 |
| Resolutiona (Å) | 62.7–1.75 (1.79–1.75) | 44.0–3.3 (3.4–3.1) |
| No of reflectionsa | 52290 (3535) | 35872 (2508) |
| Atoms protein/water | 3398/567 | 13302/290 |
| No test set reflections | 2753 | 3580 |
| Rwork/Rfreea (%)a | 16.3/18.5 (21.7/22.0) | 22.1/26.0 (25.0–31.5) |
| r.m.s.d.bonds (Å)/angles (°) | 0.007/1.27 | 0.009/1.25 |
| B-average | 14.2 | 44.4 |
| Ramachandran, Preferred, | 96.2/3.8/0 | 90/6.6/3.4 |