Figure 2.

Effects of metals on the formation of acid organelles. Pollen tubes were cultured in standard medium (A), or treated with 10 μM CdCl₂ (B), 20 μM CuCl₂ (C), 2 μM Na₂HAs₄O.12H₂O (D), 20 μM CrCl₃ (E), and 5 μM HgCl₂ (F), respectively, for 20 h. All samples were stained with 10 μM Lyso-Tracker Green (Invitrogen, final concentration) for 30 min, and then were examined using a Zeiss 5 Live laser scanning confocal microscope, with an excitation wavelength of 488 nm and emitted wavelength of BP 494–555 nm. Bars = 10 μm.