Figure 1.

Gli2-specific shRNAs suppressed the expressions of Gli2 mRNA and protein in SMMC-7721 cells. (A) The level of Gli2 mRNA was assessed by semi-quantitative RT-PCR. Total RNA was extracted from SMMC-7721-shRNA, SMMC-7721-NC, SMMC-7721. GAPDH was used as a normalization control. (B) The level of Gli2 protein expression was assessed by Western blot. β-actin was used as a loading control. Densitometric analysis was performed using Quantity one 4.6.2 software. *P < 0.05 compared with control. These experiments were performed in triplicate.