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. 2015 May 6;16(6):941–948. doi: 10.1080/15384047.2015.1040963

Figure 1.

Figure 1.

MiR-15a and miR-16 increase autophagic activity. (A, B) MiR-15a and miR-16 promote GFP-LC3 puncta formation. HeLa cells stably expression GFP-LC3 were transfected with miR-15a, miR-16 or negative control (NC). Cells were fixed at 48 h post transfection. (A) Representative images were captured by confocal microscope. Scale bar, 10 μm. (B) Quantitative analysis of GFP-LC3 puncta in (A). Data shown are means ± SD of three independent experiments. **P < 0.01. (C) Over-expression of miR-15a or miR-16 induces LC3-II conversion and p62 degradation. Densitometric analysis were calculated using Image J software. (D) Overexpression of miR-15a and miR-16 increase autophagic flux. HeLa cells transfected with NC, miR-15a or miR-16 were treated with Baf A1 (10 nM) for 2 h. Western blotting was performed to analyze the status of LC3, p62 and GAPDH. (E) Densitometric analysis of LC3-II/GAPDH or (F) p62/GAPDH ratios from immunoblots using Image J. Data shown are means ± SD of three independent experiments. *P < 0.05, **P < 0.01, student 2-tailed t test.