Figure 3.

Rictor is a direct target of miR-15a and miR-16. (A) Predicted binding sequence of miR-15a, miR-16 in Rictor 3′ UTR. (B) Western blot analysis of Rictor and GAPDH proteins in HeLa cells transfected with miR-15a, miR-16, NC, ant-miR-15a, ant-miR-16 or anti-NC. Protein ratios were determined by ImageJ densitometric analysis. (C) Luciferase reporter assay of wild type (WT) or mutated (MUT) Rictor 3′UTR vector co-transfected with NC, miR-15a or miR-16 respectively. Data shown are means ± SD of three independent experiments, **P < 0.01, student 2-tailed t test. (D) HeLa cells were transfected with Rictor siRNAs, miR-15a, miR-16 or NC. Western blotting was performed to analyze the status of LC3, p62, Rictor and GAPDH.