Figure 2.

Enhanced temozolomide-induced toxicity in hPMC2 downregulated MDA-MB-468 cells. Control and hPMC2 downregulated MDA-MB-468 cells were analyzed via Western blot for hPMC2 levels. (A) Proteins were extracted from cells and processed for western blot analyses. Blots were probed with actin as a loading control. hPMC2 expression in control cells was given an arbitrary value of 1 and hPMC2 expression in downregulated cells was plotted relative to control. Error bars indicate SEM of 3 independent experiments. (B) Control and hPMC2 downregulated MDA-MB-468 cells were either untreated or treated with 125 μM TMZ for 24 hours. Genomic DNA was isolated from cells and a biotinylated ARP was used to detect the abasic sites. Error bars indicate SEM of 2 independent experiments, with triplicate values for each treatment. a, significance (P < 0.05) vs. untreated cells; b, significance (P < 0.05) vs. control transfected cells with the same treatment. (C) Control and hPMC2 downregulated MDA-MB-468 cells were either untreated or treated with 125 μM TMZ for 48 hours and maintained for a week. MTT assay was performed and the bars represent the fold change in % survival. Error bars indicate SEM of 2 independent experiments with triplicates for each treatment. a, significance (P < 0.05) vs. untreated cells; b, significance (P < 0.05) vs. control transfected cells with the same treatment.