Figure 1.

Anti-proliferative activity of cucurbitacin in glioma cell lines and non-neoplastic astrocytes. EGFR overexpressing human glioma cell lines U87-EGFR-WT, U87-EGFRviii and isogenic control, U87 (A), primary cultured glioma cells, GBM-1, GBM-8, GBM-9 and GBM-13 (B) and non-neoplastic human astrocytes (C) were grown on 96-well plates in growth medium and, after an overnight attachment period, were exposed to selected concentrations of inhibitor or vehicle (0, DMSO) for 24, 48, or 72 h (Day 1, Day 2 or Day 3). Cell proliferation inhibition was assessed semiquantitatively by spectrophotometric measurement of MTS bioreduction. Points represent the mean of 3 measurements carried out in triplicate.