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. 2014 Nov 21;16(2):233–243. doi: 10.4161/15384047.2014.987548

Figure 2.

Figure 2.

Cucurbitacin inhibits Aurora kinase A, Aurora kinase B and survivin. U87 and U87-EGFRviii (A and B) cells were treated with cucurbitacin (indicated concentration) for 24 h. (C), A panel of 4 glioma cell lines (U87, LN229, T98G and LNZ308) along with EGFR over expressing U87-EGFR-WT (U87-EGFR) and U87-EGFRviii cells were treated with cucurbitacin (indicated concentration) for 24 h. Cell extracts were subjected to Western blot analysis with indicated antibody. (D). U87 cells were treated with cucurbitacin (indicated concentration) for 24 h, after which total RNA was extracted and Aurora kinase A, Aurora kinase B and survivin mRNA levels were quantified by real time PCR as described in the Materials and Methods.