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. 2014 Nov 21;16(2):233–243. doi: 10.4161/15384047.2014.987548

Figure 3.

Figure 3.

Cucurbitacin induces G2/M arrest regardless of EGFR/PTEN/p53 status in malignant human glioma cell lines. U87, U87-EGFRviii, T98G, LN18, LNZ308, A172 and LN229 cells were seeded at 60% confluence, allowed to attach overnight, and treated with cucurbitacin (indicated concentration) for 24 h. Control cells received DMSO (0). Cell cycle analysis using propidium iodide (PI) staining was performed as described in the Materials and Methods Section. Representative results of 3 independent experiments are presented here (A). Histogram represents the average of 3 experiments (B). LN18, A172 and LN229 cells were seeded at 60% confluence, allowed to attach overnight, and treated with cucurbitacin (indicated concentration) for 48 or 72 h. Control cells received DMSO (0). Cell cycle analysis was performed as described in the Materials and Methods Section (C).