Figure 5.

Targeting Aurora kinase A, Aurora kinase B and survivin by RNA interference enhanced ABT-737-induced cytotoxicity. U87 cells were transfected with non-target or Aurora kinase A, Aurora kinase B or survivin shRNA as described in the Materials and Methods. Forty-eight hours post-transfection, cells were treated with the indicated concentrations of ABT-737 for 24 h, and viability was assessed by annexin V/PI apoptosis assay (right panel; ** P < 0.005 versus non-target shRNA control). In parallel, cell lysates were collected and protein was subjected to Western blot analysis using indicated antibodies. Immunoblots were stripped and reprobed with β-actin (left panel). Data are representative of triplicate studies from 3 independent experiments (**, P <0.005).