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. 2015 Feb 13;16(3):438–449. doi: 10.1080/15384047.2014.1003005

Figure 6.

Figure 6

(See previous page). Effect of TH-302 (T) in combination with gemcitabine (G) and nab-paclitaxel (nP) on tumor microenvironment. PANC-1 tumor bearing animals received T, 50 mg/kg, ip; G, 60 mg/kg ip; and nP 30 mg/kg, iv, at a Q3Dx5 regimen. Tumors were collected 24 hrs after the last treatment. (A), representative images of Picrosirius red histology staining, α-smooth muscle actin (α-SMA) and pimonidazole immunostaining in vehicle (V), T alone, G + nP doublet and G + nP + T triplet groups. Morphometric analysis of (B) percentage of extracellular collagen by Picrosirius red staining; (C) percentage of α-SMA positivity inside the tumor; and (D) hypoxic fraction in the whole tumor by pimonidazole immunostaining. *, p < 0.05 as compared to Vehicle (V). a, P < 0.05 as compared to G + nP; b, P < 0.05 as compared to T alone. (E), representative images of pimonidazole and Caspase-3 immunostaining on consecutive sections. H, hypoxic compartment; O, oxic compartment; N, necrotic region; arrow, indicating the positive cells.