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. 2014 Oct 30;6(6):1560–1570. doi: 10.4161/19420862.2014.975099

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© 2014 Taylor & Francis Group, LLC

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Figure 3. — Increasing the valency of DR5 Nanobodies results in more efficient DISC recruitment. (A) MIA PaCa-2 cells treated with 1 nM of APO2L/TRAIL, DR5Nb1-tri, -tetra or -penta for the indicated times and assayed for DISC recruitment by measuring FADD and caspase-8 levels by Western blot following IP for DR5. (B) MIA PaCa-2 cells were treated with 1 nM x-LBY135 or DR5Nb1-tetra for the indicated times before DISC analysis following IP for DR5. (C) An extended time course of x-LBY135 treatment. (D) MIA PaCa-2 cells were treated with 1 nM of APO2L/TRAIL, DR5Nb1-tri, -tetra or -penta for the indicated times before DISC analysis following IP for Caspase-8. (E) BxPC-3 treated with 1 nM pre-crosslinked LBY135 or DR5Nb1-tetra and assayed for DISC recruitment following IP for DR5.