Table 1.
Affinities of monomeric and trivalent DR5-targeting Nanobodies determined by surface plasmon resonance and IC50 of trivalent Nanobodies in cell survival assay with COLO 205 colon cancer cell line. Monomeric Nanobodies are inactive
| Nanobody | Monomer KD (M) | Trimer KD (M) | Monomer IC50 (M) | Trimer IC50 (M) |
|---|---|---|---|---|
| DR5Nb1 | 2 × 10−9 | 2 × 10−10 | >20 | 4.6 × 10−11 |
| DR5Nb2 | 2 × 10−10 | 5 × 10−11 | >20 | 3.8 × 10−11 |
| DR5Nb3 | 1 × 10−9 | 3 × 10−11 | >20 | 1.1 × 10−10 |
| DR5Nb4 | 7 × 10−9 | 3 × 10−12 | >20 | 1.2 × 10−10 |
| DR5Nb5 | 4 × 10−9 | 5 × 10−11 | >20 | 4.0 × 10−11 |