Figure 4.

Down-regulation of USP22 expression reduces cellular viability and induces G0/G1 cell cycle arrest in NPC cells. (A) MTS assay and cell counting assay were performed to analyze proliferation viability and growth viability of CNE-2 and CNE-1 stable cell lines (B), respectively. The result showed that downregulation of USP22 expression inhibits cellular cell growth and. Proliferation. (C) USP22 expression silencing arrested CNE-2 cells and CNE-1 cells (D) in the G0/G1 phase, as determined by flow cytometric analysis. Each independent experiment was repeated 3 times. All experiments were performed in triplicate and results are expressed as means ± SD analyzed with ANOVA. **: P < 0.01