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. 2014 Nov 20;16(1):88–96. doi: 10.4161/15384047.2014.987029

Figure 5.

Figure 5.

Konckdown of USP22 expression inactivates AKT/GSK-3/Cyclin signaling pathway. (A) Western blot was used to analyze the expression of USP22, Bmi-1, PTEN, p-AKT, AKT, p-GSK-3β, GSK-3β, and cyclinD1 in the CNE-2 and CNE-1 cell lines. GAPDH serve as a loading control. (B) Analysis the gray level of western blot results using ImageJ. The relative protein levels were indicated as bars. All experiments were performed in triplicate and results are expressed as means ± SD analyzed with ANOVA. **: P < 0.01 vs CNE-2 NC, *: P < 0.05 vs CNE-2 NC, ##: P < 0.01 vs CNE-1 NC, #: P < 0.01 vs CNE-1 NC.