Table 3.
Differentially expressed proteins in Hymenaea plants after heat stress as identified by 2-D gel electrophoresis
| SN | Spot# | Proteins | Fold change* |
|---|---|---|---|
| 1 | 99 | Oxygen-evolving enhancer protein 2, chloroplastic OS = Solanum tuberosum | 28.5 |
| 2 | 97 | Oxygen-evolving enhancer protein 2, chloroplastic OS = Solanum tuberosum | 26.2 |
| 3 | 126 | Histone H3-like 5 OS = Arabidopsis thaliana | 19.1 |
| 4 | 47 | glutamine synthase [Gossypium hirsutum] | 13.6 |
| 5 | 129 | Photosystem I reaction center subunit II, chloroplastic OS = Solanum lycopersicum | 11.5 |
| 6 | 109 | Germin-like protein [Pisum sativum] | 9.8 |
| 7 | 49 | glutamine synthetase isoform GSe1 [Triticum aestivum] | 8.1 |
| 8 | 132 | Ras-related protein RABB1b OS = Arabidopsis thaliana | 8.0 |
| 9 | 70 | Oxygen-evolving enhancer protein 1, chloroplastic OS = Spinacia oleracea | 7.5 |
| 10 | 21 | Ribulose bisphosphate carboxylase large chain (Fragment) OS = Aesculus pavia | 7.4 |
| 11 | 127 | Histone H2B.3 OS = Arabidopsis thaliana | 7.2 |
| 12 | 98 | 20 kDa chaperonin, chloroplastic OS = Arabidopsis thaliana | 6.2 |
| 13 | 6 | PREDICTED: ATP-dependent zinc metalloprotease FTSH, chloroplastic [Vitis vinifera] | 5.9 |
| 14 | 46 | Fructose-bisphosphate aldolase 1, chloroplastic (Fragment) OS = Pisum sativum | 5.8 |
| 15 | 87 | caffeoyl-CoA-O-methyltransferase [Leucaena leucocephala] | 5.6 |
| 16 | 50 | Fructose-bisphosphate aldolase 1, chloroplastic (Fragment) OS = Pisum sativum | 5.6 |
| 17 | 18 | ATP synthase subunit α, chloroplastic OS = Manihot esculenta | 5.5 |
| 18 | 55 | Isoflavone reductase, putative [Ricinus communis] | 5.3 |
| 19 | 110 | Putative protein phosphatase 2C 22 OS = Oryza sativa subsp. japonica | 5.1 |
| 20 | 52 | ATP synthase gamma chain 1, chloroplastic OS = Arabidopsis thaliana | 4.9 |
| 21 | 133 | Ribulose bisphosphate carboxylase small chain 3, chloroplastic OS = Solanum tuberosum | 4.7 |
| 22 | 25 | Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic OS = Hordeum vulgare | 4.4 |
| 23 | 103 | Carbonic anhydrase OS = Flaveria brownii | 4.4 |
| 24 | 105 | (3S,6E)-nerolidol synthase 1 OS = Fragaria ananassa | 4.2 |
| 25 | 43 | Sedoheptulose-1,7-bisphosphatase, chloroplastic OS = Spinacia oleracea | 4.2 |
| 26 | 104 | ATP synthase delta chain, chloroplastic OS = Spinacia oleracea | 3.7 |
| 27 | 20 | ATP synthase subunit β, chloroplastic OS = Morus indica GN = atpB | 3.7 |
| 28 | 10 | Stromal 70 kDa heat shock-related protein, chloroplastic OS = Pisum sativum | 2.2 |
| 29 | 66 | Ribulose bisphosphate carboxylase large chain (Fragment) OS = Sinapis alba | 2.6 |
| 30 | 96 | 2-Cys peroxiredoxin BAS1-like, chloroplastic OS = Arabidopsis thaliana | −2.1 |
| 31 | 77 | BTB/POZ domain-containing protein At5g48130 OS = Arabidopsis thaliana | −2.0 |
| 32 | 83 | Oxygen-evolving enhancer protein 1–2, chloroplastic OS = Arabidopsis thaliana | −2.0 |
| 33 | 64 | Ferredoxin–NADP reductase, leaf isozyme, chloroplastic OS = Pisum sativum | −2.1 |
| 34 | 81 | Ribulose bisphosphate carboxylase large chain OS = Carica papaya | −2.1 |
| 35 | 23 | Enolase 2 OS = Hevea brasiliensis | −2.1 |
| 36 | 63 | lyase [Streptomyces sviceus ATCC 29083] | −2.2 |
| 37 | 13 | Ribulose bisphosphate carboxylase large chain (Fragment) OS = Tasmannia insipida | −2.3 |
| 38 | 72 | Coproporphyrinogen-III oxidase, chloroplastic OS = Oryza sativa subsp japonica | −2.3 |
| 39 | 128 | Peptidyl-prolyl cis-trans isomerase OS = Catharanthus roseus | −2.4 |
| 40 | 90 | Triosephosphate isomerase, chloroplastic OS = Spinacia oleracea | −2.6 |
| 41 | 65 | Isoflavone reductase homolog OS = Solanum tuberosum | −2.8 |
| 42 | 1 | Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic OS = Vigna radiata var. radiata G | −2.8 |
| 43 | 124 | Ribulose bisphosphate carboxylase large chain (Fragment) OS = Humiria balsamifera | −3.0 |
| 44 | 89 | Triosephosphate isomerase, cytosolic OS = Zea mays | −3.1 |
| 45 | 24 | Glutamate–glyoxylate aminotransferase 1 OS = Arabidopsis thaliana | −3.6 |
| 46 | 3 | Chaperone protein ClpC, chloroplastic OS = Pisum sativum | −3.9 |
| 47 | 74 | Ribulose bisphosphate carboxylase large chain (Fragment) OS = Sinapis alba | −3.9 |
| 48 | 69 | Ribulose bisphosphate carboxylase small chain, chloroplastic OS = Glycine tabacina | −4.1 |
| 49 | 14 | Ribulose bisphosphate carboxylase large chain (Fragment) OS = Humiria balsamifera | −4.1 |
| 50 | 16 | Ribulose bisphosphate carboxylase large chain (Fragment) OS = Pachira aquatica | −4.2 |
| 51 | 92 | Carbonic anhydrase OS = Flaveria bidentis | −4.4 |
| 52 | 8 | Ribulose bisphosphate carboxylase large chain OS = Viscum album | −4.5 |
| 53 | 34 | Phosphoglycerate kinase, chloroplastic OS = Nicotiana tabacum | −5.0 |
| 54 | 135 | putative cold-inducible protein-like protein [Helleborus orientalis] | −5.1 |
| 55 | 41 | mucunain [Mucuna pruriens] | −7.8 |
| 56 | 119 | Probable protein arginine N-methyltransferase 6.1 OS = Oryza sativa subsp indica | −9.4 |
| 57 | 82 | Endochitinase A2 OS = Pisum sativum | −23.7 |
*
Average ratio of 3 biological replications (Heat/control). “−”sign denotes down-regulation of particular protein expression.