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The “cut-and-paste” process of integrating gene(s) of interest into host genome. (A) SBase, whose expression driven by the promoter, recognizes the IR/DR sequence of the transposon and binds to these sites. (B) The synaptic complex formatted. (C) SBase tetramers cut the donor sequence between IR/DR sites. (D) SBase tetramers recognize target sites in the genome and bind to it. (E) The gene(s) of interest between IR/DR sites reintegrate into the genome.
