Table 1.

SB transposon system used for the construction of cancer mouse models

Transposon insert element	Cancer type	Method	Animals	Ref.
T2/Onc3	Squamous cell carcinoma, HCC	Pro-nuclear injection, knock in ES cell technology, ES cell electroporation, hybridization	C57BL/6J mice and C57BL/6J C3H hybrid mice	20
T2/Onc	HCC	Pro-nuclear injection, knock in ES cell technology, hybridization	Hepatocyte-specific Alb-Cre mice, Rosa26-lsl-SB11 mice and p53-lsl-R270H mice	29
T2/Onc2	Intestinal intraepithelial neoplasia, adenocarcinoma, and adenoma	Pro-nuclear injection, knock in ES cell technology, hybridization	Rosa26-lsl-SB11 mice, Villin-Cre mice, and T2/Onc mice	30
T2/Onc2	Skin cancers	Pro-nuclear injection, hybridization	C57BL/6J × DBA/2J F2 embryos, AC heterozygous mice	57
T2/Onc	Liver cancer	Pro-nuclear injection, knock in ES cell technology, hybridization	Tet-on-MYC mice, LAPtTA mice, Rosa26-SB11 mice, and T2/Onc mice	58
T2/Onc2 and T2/Onc3	Pancreatic adenocarcinoma	Pro-nuclear injection, knock in ES cell technology, hybridization	LSL-KrasG12D, Pdx1-Cre, T2Onc2, T2Onc3, and Rosa26-LSL-SB11 transgenic mice	59
N-RAS	liver cancer	Hydrodynamic tail vein injection	C57BL/6J p19Arf-null mice	62
H-RAS, c-Myc, shp53	Sarcomatoid carcinoma	Subcutaneous injection	C57BL/6 female mice	63
Fah, HBx, shp53 and N-RAS	HCC	Hydrodynamic tail vein injection	Fah−/− mice	72

Alb, Albumin; c-Myc, v-myc avian myelocytomatosis viral oncogene homolog; CRC, colorectal cancer cell; Cre, a cyclization recombination enzyme; ES cell, embryonic stem cell; Fah, fumarylacetoacetate hydrolase gene; HCC, hepatocellular carcinoma; H-RAS, Harvey rat sarcoma virus oncogene; Kras, Kirsten rat sarcoma viral oncogene homolog; LAPtTA, a liver-specific tet-transactivator protein; lsl, loxP-stop-loxP; N-RAS, neuroblastoma ras oncogene; p19Arf, a positive regulator of the p53 tumor suppressor, and loss of Arf predisposes to a wide spectrum of tumors; Pdx1, pancreatic and duodenal homeobox 1; p53-lsl-R270H, R270H targeted point mutations of p53, which is considered as a conditional dominant negative p53 transgene; Rosa26-lsl-SB11, SB11 transposase cDNA preceded by lsl knocked into the Rosa26 locus; tet-o-MYC, tetracycline-repressible MYC transgene; shp53, a short hairpin RNA against tumor suppressor Trp53 encoding gene.