Figure 6.

Histopathological photomicrographs of pancreatic tumors in diabetic and normal nude mice. (A) Hematoxylin and eosin staining and immunostaining for a primary anti-NF200 antibody of sections for nerves and PanCa cell tumor sections. The tumor tissue penetrated the nerve tissue in an invasive manner (original magnification, ×100). The tumor diameters were larger (B) in the diabetic mice than in the normal mice. (C) In the electron micrographs of sciatic nerve invaded by pancreatic tumors, PanCa cells exhibited irregular nuclei (arrow) as well as mass-like accumulations and large nucleoli (arrow). Their mitochondrial structure was normal (arrow). The interspace between the nerve fiber and lamellar sheath increased significantly (arrow). The lamellar sheath segmentally dissolved (arrow), and extensive SC apoptosis in sciatic nerve of DM was detected. The layered structure of the myelin sheath was loose and had dissolved in the right and left limbs of mice in the diabetic group. In addition, the neurites remained intact in the right hind limb (arrow) but dissolved in the left hind limb (arrow).