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. 2015 Jan 22;16(1):115–124. doi: 10.4161/15384047.2014.987070

Figure 2.

Figure 2.

Overexpression of pre-miR-202 blocked the expression of its target protein Mxd1 mRNA and decreased Mxd1-3′-UTR activity. (A, B) Exposure of PANC-1 cells to 3-Cl-AHPC increased Mxd1 and SAP18 proteins levels as demonstrated by Western blots analysis and miR-578 miRNA mimic decreased SAP18-3′-UTR luciferase activity in cells (C, D). Increased expression of pre-miR-202 expression vector and pre-miR-202 reduced expression of Mxd1 mRNA in pre-miR-202 stably transfected cells. (D) Over-expression of pre-miR-202 blocked 3-Cl-AHPC mediated mRNA expression of Mxd1. (E) miR-202 decreased Mxd1-3′-UTR activity in transiently transfected cells. Error bars represent the mean of 3 separate determinations ± the standard deviation (SD). *, ** and *** (<0 .05, <0 .01 and <0 .001) were significantly differences between control and treated samples and •• was significantly different between 3-Cl-AHPC treated vector and pre-miR-202 expressing cells using the t-Test.