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. 2015 Jan 22;16(1):115–124. doi: 10.4161/15384047.2014.987070

Figure 6.

Figure 6.

hTERT depletion is essential for induction of apoptosis in PANC-1 cells. (A) Knockdown of TERT led to nuclear fragmentation at 72 h of transfection in PANC-1 cells as determined by Phase Contrast Microscopy. (B) hTERT mRNA expression decreased in stably transfected sh-TERT knockdown cells. (C) sh-RNA-TERT knockdown cells induced apoptosis in transiently transfected cells. (D) Overexpression of hTERT blocked 3-Cl-AHPC mediated apoptosis. The apoptosis induction was measured by flow cytometry using Annexin V-FITC binding together with propidium iodide (PI) staining. Percentage of apoptotic cells corresponds to lower right (early apoptotic cells, annexin V positive, PI-negative) quadrants (right panel). (E) Increased hTERT mRNA expression after 72 h in over-expressing hTERT cells. Error bars represent the mean of 3 separate determinations ± SD. * and *** (<0 .05, and <0 .001) indicate significantly differences between vector and sh-TERT knockdown cells and ♦♦ (<0 .01) indicate significantly difference apoptosis between 3-Cl-AHPC treated and hTERT overexpressing cells using the t-Test.